ABSTRACT
Micro-terrorism! We coin this term to show the horrible face of microbiology in a way similar to the current widespread terrorism throughout the world. Throughout the ages, tiny organisms were the immediate cause of devastating epidemics that killed hundreds of millions of the inhabitants of the planet. Corona virus disease-19 (COVID-19) is a prominent milestone in the history of microbiology. Historically, microbiology witnessed prominent milestones from the discoveries of Antoni Philips van Leeuwenhoek and Louis Pasteur to the development of antibiotics in the first half of the twentieth century. Nowadays we live a new milestone in microbiology history. From the public point of view, it is astonishing that a tiny unseen organism could produce such a huge transition in the history of the world. Extra jeopardy is that the COVID-19 patient put his contacts at work and household members at a real risk of rapidly transmitting the disease all over the world. © 2020 The author (s). Published by Zagazig University.
ABSTRACT
Selected monoclonal antibody molecules were conducted using the antibody-antigen docking mode, as well as the antibody-antigen docking approach. The objective of the study was to check the effects of Cetuximab COVID-19 proteins (Nsp15 and 3CLpro) by using antibody-antigen docking mode, as well as the antibody-antigen docking approach. The results of molecular docking revealed that Cetuximab, a cancer-fighting antibody, ranks first among antibodies to both COVID-19 proteins (Nsp15 and 3CLpro). In cetuximab-3CLpro and cetuximab-Nsp15 complexes, the antigen interacts with both antibody chains, H and L. According to the findings, Cetuximab can be added to the COVID-19 treatment protocol, which may have the desired effect of inhibiting viral replication and decreasing mortality by targeting COVID-19 proteins (Nsp15 and 3CLpro). Validation of these computational findings will require additional in vitro and in vivo research, which can be considered as a contribution in the field of biotechnology © 2023, Physical Chemistry Research.All Rights Reserved.
ABSTRACT
Two active coronaviral proteins (3CLpro and Nsp15) have been studied using both the GC-MS and docking methods. These coronaviral proteins have been examined with the methanol extract generated from leaves of the Arum palaestinum. According to the GC-MS findings, 19 major natural compounds are present in the plant’s methanolic extract. The lowest Binding Energy (LBE) and the inhibition constant (Ki) have been used to identify and classify the potential of these lead drugs with their pharmacological properties. The affinity of these compounds with coronaviral proteins has been evaluated to reveal the usage of these compounds at the active sites of the receptors, 3CLpro (PDB ID: 6LU7) and Nsp15 (PDB ID: 6VWW). The results of β-Sitosterol, Androstan-3-one, Phenobarbital, Maltose, and α-Tocopherol show more affinity to Nsp15 and 3CLpro than to the supporting control drugs. Furthermore, an evaluation of the interactions of these components with the amino acids of 3CLpro and Nsp15 revealed that β-Sitosterol has the best LBE score and Ki value as compared with those of the approved medication and all other compounds under investigation. Consequently, these potential compounds may be modern inhibitors of coronavirus. Further in vitro and in vivo studies are needed for such computational findings. © 2021 by Polish Pharmaceutical Society. This is an open-access article under the CC BY NC license (https://creativecommons.org/licenses/by-nc/4.0/).
ABSTRACT
Today, information technology is everywhere and accessible to almost all. In this age of information technology, medical education is now faced with novel challenges. The online healthcare information boom, on the one hand constantly challenges medical students to update and extend their current body of knowledge quickly. The fast expansion of higher education online learning has also benefited from the possible cost savings for unlimited learners. Passively arranged into an experimental or control group were the undergraduates who took part in the included experiments. The main of this study is to compare online and offline exams. Methods: Data was collected from the examination department of college of Medicine, Najran University. Data was entered in the SPSS ver.20 for analysis. Descriptive and inferential statistics was obtained. (Mean, scores, frequencies and percentages), T test and chi-square tests were used to find out the significant differences and degree of associations between the scores and item analysis parameters. Results: Out of 6 tests, we did not observe the significant differences among the online and offline pass rat except Exam F (P<0.01), Easy questions are significantly increased in online exams. Conclusion: We evaluated the effect of the sudden change in the evaluation as the product of the pandemic of COVID-19. Licensed Online MCQs More accurate, greater ability to discriminate, but easier than Paper-based test. The success of the general learner in theoretical and functional assessments have been greatly improved in on-line review. © 2020 Lahore Medical And Dental College. All rights reserved.